Journal: International Journal of Surgery (London, England)
Article Title: Integrative analysis identifies molecular features of fibroblast and the significance of fibrosis on neoadjuvant chemotherapy response in breast cancer
doi: 10.1097/JS9.0000000000001360
Figure Lengend Snippet: Identifying npCR-associated fibroblasts by integrating proteomics and single-cell sequencing data. (A) Volcano plot of differentially expressed proteins between the pCR and npCR groups. (B) Upregulated pathways in the npCR group based on GO term enrichment analysis of differentially expressed proteins. (C–D) Uniform Manifold Approximation and Projection (UMAP) of major cell populations in our study, different colored dots represent different cell types. (E–F) The pCR and npCR phenotypes were mapped from proteomics to scRNA-seq data. (G) Pseudotime trajectories of fibroblast. (H–I) The GO/KEGG enrichment based on the GSVA algorithm. (J) Transcription factor regulation of npCR-related fibroblast compared with other fibroblast. (K–L) The intercellular cross-talk analysis between fibroblast and other cells in TME.
Article Snippet: The spatial transcription dataset (Fresh Frozen Visium on CytAssist: HumanBreast Cancer, Probe-Based Whole Transcriptome Profiling) was retrieved from 10X Genomics ( https://www.10xgenomics.com/ ).
Techniques: Sequencing